Gene Expression of Mitochondrial Enzymes from Exercise

Gene Expression of Mitochondrial Enzymes from Exercise Induced Change in Gene Expression of Mitochondrial Enzymes as a Result of Intense Exercise Abstract Exercise-induced changes in gene expression of mitochondrial enzymes has become a leading target for sports medicine research. Previous indirect testing of delayed-onset muscle soreness and changes in rotation of motion do not provide a sufficient explanation of the induced changes to the genome (Hubal, et al., 2010). Biomarker testing has allowed for detecting levels of proteins within a sample. Blood and serum levels, taken before and after exercise, have been analyzed and tested for changes in protein activity. Calf-raises show an increase in creatine kinase (CK) and aldolase (ALD) activities (Kanda, et al., 2014). MicroRNA arrays along with telomere extension mRNA arrays and quantitative real-time PCR on RNA taken from white blood cells have shown to down-regulate telomeric repeat binding factor 2 (Chilton, et al., 2014). Biochemical testing at the genomic level will provide a better understanding of the long-term effects of intense exercise. Knowing these high-intensity induced gene expressions in mitochondrial DNA aids in knowing what causes diseases such as Rhabdomyolysis. Introduction Centuries ago staying physically fit was accentual to stay alive. Those that were not fit were not able to kill prey and therefore would be less likely to survive. In today’s world, being physically fit is not a necessity, but rather something that many people strive for to live a healthy lifestyle. Living an active life lowers the chance of many diseases, such as kidney and Alzheimer’s, and cancers, such as colon and breast. It is therefore crucial to understand the biochemistry behind exercise as a helpful preventative measure for health problems. When exercising, the body is put through tasks that disrupt homeostasis. The body wants to eliminate wide spread deviants of homeostasis. However, after and during exercise the body needs more oxygen and energy to be able to complete the tasks one is putting on the body. These demands, the increase in affinity for oxygen and energy, require metabolic responses that disrupt homeostasis. To test these metabolic responses, many scientists use biomarker testing on whole blood and serum samples rather than pieces of skeletal muscle. Biomarkers are used to measure the presence of a physiological state. These markers have biological properties that measure the blood and serum. There are many different changes in the mitochondrial genome during and immediately following exercise. This paper will focus on an overview of some endurance training biomarkers, but will mainly focus on high intensity exercise and the induced gene expression in the mitochondrial genome. It is important to study the effects of exercise on gene expression to know at what levels of various genes, like lactate dehydrogenase, aldose, etc., come dangerous to human health as to prevention diseases such as Rhadomyolysis (Heled, et al., 2005). Proliferator-activated Receptors association with Exercise-Induced Mitochondrial Biogenesis Transcriptional cofactors peroxisome proliferator-activated receptors (PGC) regulate gene expression (Lin, et al., 2005). PGC-1a regulates gene expression in the mitochondrial genome. This coactivator interacts with other proteins to regulate contraction. Overexpression of PGC-1a results in an increase in mitochondrial gene function (Lin, et al., 2002). Calcium/Calmodulin-dependent kinase 2 (CaMKII), AMP-activated protein kinase (AMPK), and nitrogen-activated protein kinases (MAPKs) all have important signaling that help regulate PGC-1a (Hawley, et al., 2010). Calcium is important for energy due to its role in the sarcoplasmic reticulum. Contractions of skeletal muscle are dependent on high levels of calcium. The calcium then binds to troponin, which moves the myosin fibers resulting in muscle contractions. Calcium also plays a major role in calcium-calmodulin-dependent kinases. Calcium/Calmodulin-dependent kinase 2 (CaMKII) activation increases the transport of glucose in skeletal muscle (Rose, A. and Hargreaves, M., 2003). AMPK and MAPks activate PGC-1a, Figure 1, by phosphorylating transcription factors myocyte enhancer factor 2 and ATF-2, respectively (McGee and Hargreaves, 2010). Figure 1. Schematic of the Major Signaling Pathways Involved in the Control of Skeletal Muscle Hypertrophy and Mitochondrial Biogenesis (Hawley, et al., 2010). The right side of this figure shows the correlation of the AMPK, CAMK, SIRT1, MAPK pathways that effect PGC-1a which regulates mitochondrial biogenesis. Another inhibitor of PGC-1a is a deactelyase silent mating type-information regulation 2 homolg 1 (SIRT1) (HIgashida, et al., 2013). Since SIRT1 is NADà ¢Ã‚ Ã‚ º-dependent, Figure 1, changes in concentration of NADà ¢Ã‚ Ã‚ º change the SIRT1 activity in the cell (Gurd, 2011). Many studies have shown that the protein content of SIRT1 and the activity are independent, and that because of that it is thought that the SIRT1 activity is what subsidizes to PGC-1a activity (Gurd 2011). Test For Correlation between SIRT1 and PGC-1a. The following experiment was performed by Chabi and coworkers to examine SIRT1’s role in muscle during muscle use (Chabi, et al., 2009). Rats were placed into a control group and a running group. The running group were able to run on a loaded wheel. Weight was added to the wheel during weeks one through four, but maintained a 200 g weight from weeks five through eight. The plantaris (PL) and soleus (SOL) muscles were taken as well as the tibialis anterior (TA) and extensor digitorum longus (EDL) for analysis. Once the proteins were extracted, the SIRT1 activity and deacetylase was found by fluorescence. A cycling assay was used to determine the NAD nucleotides. Immunoblotting was done to test expression of PGC-1a, cytochrome c, SIRT1 and GAPDH. SIRT1 expression showed to be the highest in the liver and slow-twitch muscle while PGC-1a immunoreactivity was highest in the heart muscle. This experiment did not show a correlation between SIRT1 and PGC-1a expression, like the scientists hoped, but it did bring up the question if SIRT1 activity is altered by acute exercise instead of high-intensity. Eccentric Exercise and Muscle Damage Markers The following was performed by Kazue Kanda and coworkers to see if eccentric exercise affects muscle damage markers (Kanda, et al., 2014). Participates in this study each performed right calf-raises on a force plate to add 0.5 Hz to each lift. With 3 min for rest, forty repetitions for 10 sets were completed with half of their weight along with the added force. Immediately following these muscle contractions, the medial and lateral gastrocnemius and soleus were measured for tenderness using a FP meter. The meter rated based on a visual analogue scale from no pain to extremely sore. The ankle was tested for range of motion (ROM) along the dosiflexion position (-20 ) to the plantar flexion position (15). Both of tenderness of the calf and the ROM was tested at 24 h increments until 168 h after the repetitive muscle contractions. After 72 h the tenderness of the right calf increased significantly (p Subsequently, samples from the blood and urine were taken both before and at various times after the exercise. Various proteins, creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), aldolase (ALD) and alanine aminotransferase (ALT) were tested from the serum. An Enzyme-linked immunosorbent assay (ELISA) was used to test for fatty acid-binding proteins (FABP). The activities of CK and AST increased significantly post 72 h. The activities of ALT and ALD also increased for each sample, however, it was not significant. LDH activity had a significant increase at 96 h after exercise, but not really before. The results for ALD did correlate with the tenderness of the medial gastrocnemius at the 72 h mark and could suggest that ALD might be a better muscle damage indicator because the other proteins tested did not correlate with tenderness. The ELISA testing on FABP resulted in no detectable changes. This experiment used two methods of testing muscle damage and had correlated results for ALD. Exercise-Induced biochemical changes in Quadriceps and Gastrocnemius in Mice The following was performed by L. Toti and coworkers to see the changes in blood lactate as well as mitochondrial enzymes as a result of two different exercises; intense activity with recovery periods and moderate activity with no recovery periods (Toti, et al., 2013). Immunoblotting and immunohistochemistry were used to assess the two muscle groups, the quadriceps and gastrocnemius, to see expression of enzymes correlated with oxidative metabolism. Mice were divided into three different groups based on similar maximal velocities. Mice with higher maximal velocities partaked in the intense activity: running at 90% for 2 min followed by 1 min of recovery. The mice with lower mamximal velocities partaked in continuous running at 60% velocity. Data was collected for 40 sessions, where each mouse ran a distance of 1000 meters. Blood samples were taken at sessions 1, 20 and 40. Blood lactate was tested and showed a decrease in both groups by session 40, with the higher-velocity group measuring significantly lower than the lower-velocity group. Immunochemistry testing resulted in an increase in response for the high-velocity mice for both the quadriceps and the gastrocnemius. This experiment showed that high-intensity training impacted the biochemistry more so than the low-velocity training. MicroRNA Expression and Telomere-Associated Genes After Acute Exercise The following was performed by W. Chilton and coworkers to see mechanisms the correlation between white blood cell (WBC) telomere length and exercise (Chiltion, et al., 2014). Participants in this study ran on a treadmill for 30 min at 80% of maximum oxygen uptake. Blood samples were taken before and immediately following the running as well as an hour post-running. MicroRNA expression arrays that could measure a whole genome, were used on the samples. TERT mRNA expression levels were then tested by qPCR. Telomerase reverse transcriptase (TERT) mRNA and Sirtuin-6 (SIRT6) were two of the genes tested. This experiment was able to show supporting evidence that the transcriptional regulation of key telomeric genes can be affected by exercise. TERT mRNA was upregulated as well as the SIRT6. The qPCR testing on TERT and SIRT6 showed the increase in binding miRNA. Chilton did express that the increases in both the SIRT6 and the TERT mRNAs could have been upregulations from the extra-telomeric pathways instead of just the telomeric roles since there was no definitive way to differentiate between the two in this experiment. It is important to understand exercise’s effects on telomeres and its corresponding proteins to gain an insight on how physical health improves telomere homeostasis, keeping the telomeres from getting too short and the cell dying. Chemokine Polymorphisms Association with Skeletal Muscle Damage The following experiment was performed by M. Hubal and coworkers to see if chemokine ligand 2 (CCL2) and chemokine receptor 2 (CCR 2) are associated with biomarkers after exercising (Hubal, et al., 2010). CCL2 is important because it recruits necessary items, such as memory T cells, dendritic cells, and monocytes, to inflammation sites in injured tissue. CCR2, the receptor molecule for CCL2, mediates with calcium mobilization. It is suggested that CCL2 and CCR3 play major roles in the repair of skeletal muscle damage. Participants performed two, 25 contractions, sets of elbow flexor muscle contractions in the non-dominant arm. It was crucial that the participants had constant maximal effort and stayed hydrated the 10 days following the exercise. Blood samples were taken and sent for genotyping. Single nucleotide polymorphisms (SNPs) known to influence the level of CCL2 proteins were analyzed using PCR. There were four SNPs from CCL2 and three from CCR2 that should high phenotype associations. The minor allele found in the SNPs was correlated with an increase in damage. This study was able to show that variations of the CCL2 and CCR2 genes are related to muscle damage markers caused by exercise. Molecular and Metabolic Changes of High-intensity Interval Training The following experiment was performed by J. Little and coworkers to assess the molecular and metabolic changes of high-intensity interval training (Little, et al., 2010). Participants performed six cycling training sessions over the course of two weeks. Each session consisted of approximately 30 min of high intensity intervals. By the last sessions, the subjects were completing 12 intervals of 60 s high-intensity cycling followed by 75 s low intensity for recovery. Biopsies from the leg were taken before and after the two week training. The muscle lysates were taken for Western blotting and enzyme activity testing. Western blotting was used to test for glucose transporter type 4 (GLUT4), PGC-1a, and SIRT1while the mitochondrial enzyme activity was tested on cytochrome c oxidase (COX). The subjects improved in both time and power, about 10%, for cycling during the two week training session. COX activity increased by 29%. PGC-1a increased by approximately 24%, however, the protein itself was did not have any genetic changes. GLUT4 content increased by 119%, while SIRT1 increased by approximately 56%. This experiment showed some changes, due to exercise, in regulators that are important in mitochondrial biogenesis. Conclusion There are many induced changes in the mitochondrial genome during and after exercise. These changes occur to help maintain cell homeostasis while the body is being put through stress during intense exercise. Blood and serum samples along with tissue extractions have provided a way to examine these changes and see how one is correlated with another (Figure 1). PGC-1a helps muscle contractions by regulating gene expression in mitochondria biogenesis. However, PGC-1a has many cofactors helping it. CaMKII, AMPK, and MAPKs all help in providing energy to the skeletal muscles by impacting the activation of PGC-1a. Although SIRT1 deactelyation inhibits PGC-1a, it still impacts the biochemistry of the body during workouts due to deactelyation. LDH activity was shown to increase hours after high-intensity exercise because LDH it is released as pyruvate is converted into lactate. ALD, which converts sugar into energy, was found to be a good indicator because in the calf-raises experiment the results from the biomarker testing as well as the tenderness testing correlated with each other. CCL2 and CCR2 were found to have changes in SNPs that corresponded to the high-intensity exercise and most likely aide in the recruiting of the memory T cells and dendritic cells to the injured tissue. High-intensity exercise, without time for recovery, would keep the body maintained at a stressful state of trying to bring it back to homeostasis. As proven in the calf-raise experiment, LDH levels decreased as the participants were able to come accustom to the exercises. The bodies were no longer in shock. If the bodies were did not become accustom, or the body was not given any time for recovery, the chances of obtaining diseases such as Rhabdomyolysis increases. Future experiments need to focus on what levels of these regulators will become dangerous. Research should be down to further understand the relationship between SIRT1 and PGC-1a. References Chabi, B., Adhihetty, P.J., O’Leary, M.F., Menzies, K.J., and Hood, D. (2009) Relationship between sirt1 expression and mitochondrial proteins during conditions of chronic muscle use and diuse. J. Appl. Physiol. 107(6):1730-1735. Chilton, W., Marques, F., West, J., Kannourakis, G., Berzins, S., O’Briend, B., and Charchar, F. (2014) Acute Exercise Leads to Regulation of Telomere-Associated Genes and MicroRNA Expression in Immune Cells. PLoS ONE. 9, 1-13. Gurd, B. (2011) Deacetylation of PGC-1a by SIRT1: importance for skeletal muscle function and exercise-induced mitochondrial biogenesis. App. Physiol. Nutr. Metab. 36:589-597. Hawley, J., Hargreaves, M., Joyner, M., and Zierath, J. (2010) Integrative Biology of Exercise. Cell 159, 738-749. Heled., Y., Zarian., A., Moran., D., and Hadad, E. (2005) Exercise induced rhabdomyolysis – characteristics, mechanisms and treatment. Harefuah 144(1):34-8. Hubal, M., Devaney, J., Hoffman, E., Zambraski, E., Gordish-Dressman, H., Kerns, A., Larking, J., Adham, K., Patel, R., and Clarkson, P. (2010) CCL2 and CCR2 polymorphisms are associated with markers of exercise-induced skeletal muscle damage. J of App Physicol 108(6), 1651-1658. Kanda, K., Sugama, K., Sakuma, J., Kawakami, Y., and Suzuki, K. (2014) Evaluation of serum leaking enzymes and investigation into new biomarkers for exercise-induced muscle damage Exerc Immunol Rev. 20, 39-54. Lin, J., Wu, H., Tarr, P., Zhang, C., Wu, Z., Boss, O., Michael, L., Puigserver, P., Isotani, E., Olson, E., Lowell, B., Bassel-Duby, R., and Spiegelman, B. (2002). Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres. Nature 418, 797-801. Lin, J., Handschin, C., and Spiegelman, B. (2005). Metabolic control through the PGC-1 family of transcription coactivators. Cell. Metab. 1, 361-370. Little, J.P., Safdar, A., Wilkin, G.P., Tarnopolsky, M.A., and Gibala, M.J. (2010) A practical model of low-volume high intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. J. Physiol. 588(6):1011-1022. McGee, S., Hargreaves, M. (2010). AMPK-mediated regulation of transcription in skeletal muscle. Clin. Sci. 118, 258-263. Rose, A.J., and Hargreaves, M. (2003). Exercise increases -calmodulin-dependent protein kinase II activity in human skeletal muscle. J. Physiol. 265, E380-E391. Toti, L., Bartalucci, A., Ferrucci, M., Fulceri, F., Lazzeri, G., Lenzi, P., Soldani, P., Gobbi, P., La Torre, A., and Gesi, M. (2013) High-intensity exercise training induces morphological and biochemical changes in skeletal muscles. Biol Sport 30(4), 301-309. Alissa Christian

Women in Psychology Paper

Women have made many contributions to the advancement of psychology, many of which have gone without notice until recent times, and some of which still goes unidentified in the field of psychology. The mention of women in the early development of psychology usually refers to them as minor contributors to a field that at one time was predominantly dominated by men. â€Å"Women of the time were subject to gender and martial prejudice† (Stipkovich, 2011). One such women who thrived in the field of psychology despite of and greatly due to the discrimination women experienced in the 1900’s is Leta Hollingworth.According to â€Å"Stipkovich (2011)†, â€Å"The remarkable path Leta Hollingworth’s life took her was instrumental in becoming a significant figure in the history of psychology of woman† (Contributions to the field of Psychology). Background Born Leta Anna Stetter, in May of 1886 in Nebraska, she was the oldest of three children. Raised on her gra ndparent’s farm after her mother’s death and fathers abandonment following the birth of her youngest sibling. â€Å"Leta Stetter received her early formal education in a one-room log schoolhouse, an education she later described as â€Å"excellent in every respect† (Miller, R.1990, para. 4). Leta graduated high school in 1902, at the age of 15 she was one of eight students in the class. In high school Leta showed a talent for creative writing which she was encouraged to develop in college. Leta enrolled and attended the University of Nebraska-Lincoln, â€Å"where she quickly achieved a campus reputation in literature and creative writing and was designated Class Poet of the Class of 1906† (Miller, R. 1990, p. 145). While attending the university Leta met and became engaged to classmate Harry Levi Hollingworth.Harry graduated from the university before Leta and decided to do his graduate studies in New York at Columbia University, Leta stayed in Nebraska to finish her undergraduate work and graduated in 1906. Unable to start a career in writing as she originally intended due to financial problems, Leta took a teaching position in Nebraska and later joined Harry in New York the two were married on December 31, 1908. Leta attempted to get a job as a teacher in New York but was denied based solely on her marital status. â€Å"This was a very frustrating circumstance for the talented and educated graduate and led to the questioning of the role women play in  society† (Stipkovich, 2011).Over time Leta went on to complete her graduate studies at Columbia receiving an M. A. in 1913, Ph. D. 1916. While completing her studies in educational psychology at Columbia Leta had an opportunity to work directly with Edward Lee Thorndike. According to Stipkovich (2011), â€Å"With the environment finally allowing her to explore her academic interests, and questions about her own existence as a married woman, she pursued the study of womenâ €™s psychology and new interests in giftedness and intelligence† (A little Background). Theoretical perspectiveLeta became interested in psychology after questioning women’s inferiority to men. After researching the works of other psychologist she found only one assertion that could be tested scientifically. This assertion was commonly known as the â€Å"variability hypothesis,† (Benjamin, L. , 1990 p. 147). Held, L. (2010), states â€Å"The variability hypothesis posited that men exhibit greater variation than women on both physical and psychological traits, in essence suggesting that men occupied both the highest and lowest ends of the spectrum on any trait and women were doomed to mediocrity† (para.4).In order to disprove this hypothesis Leta did some research at the Clearinghouse for Mental Defectives â€Å"Hollingworth believed societal roles accounted for the differences, not innate differences† (Held, L. 2010 p. 3). She conducted an exper iment over a three month time period on both men and women from a behaviorist perspective which essential proved there was no decrease in women’s performance during the course of their cycle. While teaching at Columbia Leta started to express an interest in the study of exceptional children.While working with these children she discovered most of them were averagely intelligent but suffered from adjustment problems due to adolescents. In 1928 Leta published â€Å"The Psychology of the Adolescent† once again done from a behaviorist perspective further research should children with high intellect could be problem children, causing her to ask what special programs have been developed for them in public schools? (Benjamin, L. , 1990). â€Å"She worked on assessment tools for early identification of the intellectually gifted, and inevitably her work led her to the development of educational methods for these children† Benjamin, L., 1990).Contributions to the field of psychology Leta Hollingworth is a contributor to three specific fields of psychology. Leta’s recognition of the challenges faced by women set precedent to a new field of psychology: the psychology of women. Barbaro (2002), â€Å"Because of her work, future women would not have to deal with unchecked acquisitions of innate mediocrity or menstrual disability in their pursuit of scientific eminence† (Contributions to Psychology).In 1921 Leta Hollingworth was cited in â€Å"American Men of Science† for her research on the psychology of women (Held, L. 2010 p. 15). The other field of psychology Leta Hollingworth was a great contributor to the psychology of the exceptional child which led to her much known involvement and influence in school psychology. Due to her studies on the gifted children she was able to develop methods to recognize gifted children and aide in the development of a school curriculum better meeting their needs.Hollingworth’s writings on gi fted children, special education, adolescence, and mental retardation were inspirational for over twenty years (Miller, R. , 1990). In clinical psychology she disproved the â€Å"variability hypothesis† her examination on both male and female infant craniums proved that while the males were slightly larger if a difference in variability existed it favored females (Held, L. 2010 p. 4). After the disproving of the â€Å"variability hypothesis† Leta Hollingworth worked in the field of clinical psychology part-time for twenty years.Other contribution to psychology are noted publishing’s such as â€Å"Gifted Children: Their Nature and Nurture† (1926) this book was based on the results of her study on gifted children and â€Å"Children Above 180 IQ† (1942) this was Leta Hollingworth’s last publication and was completed after her death by her husband, Harry L. Hollingworth (Held, L. 2010 p. 7). Conclusion Leta Hollingworth was a women extraordinary for her time. She not let her troublesome childhood prevent her from gaining an education instead she used her less than perfect up bring to develop a talent in creative writing.When she found herself unable to work doing what at the time seemed like her natural calling she went on to teach, only to find her marital status would prevent her from doing so. This turning point in her life was discouraging but, with the support of her husband went on to gain an education, and dismantle one of the theories that prevent her and other women of her time from equal treatment. Leta went on from their developing not only one but three types of psychology that had not yet been explored in-depth, the psychology of women, educational psychology, and the psychology of the gifted child.Her work in the field of psychology not only furthered the field it changed the way women were looked upon, and the education of children both gifted and non-gifted. Instead of becoming a victim of her era she went on to become a pioneering female psychologist of her time â€Å"were she to observe contemporary society, she would be gravely disappointed that in the past 50 years there has been so little progress in changing societal attitudes toward the gifted, and that women, particularly gifted women, still face so many impediments to achievement and recognition† (Silverman, L. K. 1992 p. 11).

The Unexpected Truth About Socialism Essay Topics

The Unexpected Truth About Socialism Essay Topics Privately owned companies have a tendency to get the ability to monopolize labor and product markets. While it is ordinarily feasible for individuals residing in a socialist country to have businesses or offer professional services directly to consumers, they are normally taxed heavily on their profits. States aren't Indestructible 3. The poorest cities in the united states. The Supreme Strategy to Socialism Essay Topics Characteristics central arguments in the capitalist financial model allows absolutely free candy. Furthermore, you can warn your audience about certain consequences that could occur if the problem under discussion isn't addressed properly. Besides these 3 components, there's another integral element which is called a thesis statement. Causes of adolescent violence and the way it can be avoided. That there's social and financial equality within this state and there will not be a discrimination based on cast e or gender. Music censorship and the way it's affecting us. The education process is changing. Ideas like universal healthcare coverage, old age pensions, and absolutely free education. The Hidden Treasure of Socialism Essay Topics In Capitalism, there's a huge gap between rich class and inadequate class because of unequal distribution of wealth rather than socialism where there isn't any such gap because of equal distribution of revenue. Socialism, on the flip side, is likewise an economic system, where the financial activities are owned and governed by the state itself. The government is not going to favour any 1 religion (secular). In Capitalism, there's no or marginal government interference that is just opposite in the event of Socialism. Marx's socialism was subjected to searching criticism recently. Capitalism leads to a regionalized financial system. It leads to the development of the economy of the country along with the creation of wealth but it advocates distinction between the haves and have-nots. It offers everyone an opportunity to make it big. Ways you can secure your fantasy job. It can be hard to think of a topic off the peak of your head. See our set of Vacation rentals. Individuals are encouraged to direct their talents in a manner that benefits themselves, including by starting a company or entering a very profitable profession. In a capitalist society all individuals can begin all kinds of business they want. If someone would like to take the opportunity on their very own company, even if it's next to one that's successful, the peril is in their own hands. If he is always working hard to make money, they most likely will, compared to a bum who can not get welfare in this type of system. How to Choose Socialism Essay Topics Whether an individual wants to get ahead of time and achieve a greater lifestyle or class it's up to them to do it. Women are given the brief end of the stick from birth and so it's inevitable they lag in the totally free sector. In theory, it seems like a terrific idea. Any negative problems that arise from the mix of human nature and competition are simply a little price to cover the immense opportunities provided by the computer system. Once allocating operate and creating work ideas there are lots of aspects to be considered. The development of the vegan way of life. Once you get your proposal essay ideas, it's time to get started writing. You will discover a range of argumentative essay topics but picking the perfect one might be the basic and the very first step to compose an influential essay. Despite the fact that you're just beginning to compose essays, you shouldn't struggle attempting to think of something to discuss. If you still struggle to find something which it is possible to write about, you always have the option to consider our professional quick essay writing service. While the absolutely free essays may give you inspiration for writing, they can't be used `as is' because they won't satisfy your assignment's requirements. Professional essay writers at 5staressays can help you, should you're looking for specialists to guide you. The success of your essay is in the appropriate selection of the topic. It is possible to support a specific idea when criticizing different facets but let the reader decide.

Ashford ECO 204 Principles of Microeconomics - 1078 Words

CLICK TO DOWNLOAD Ashford ECO 204 Principles of Microeconomics Week 1 Discussion 1: Circular Flow Diagram. Explain how the circular flow diagram relates to the current economic situation. Using the circular flow diagram, explain a way that your family interacts in the factor market and a way that it interacts in the products market. Discussion 2: Supply and Demand. Analyze how the law of demand applies to a recent purchase that you made. Describe how the product has changed in price and explain whether the price change is due to supply or demand. Did the change in price affect your decision to purchase the item? Week 2 Discussion 1: Elasticity. Analyze the determinants of the price elasticity of demand and determine if each of the†¦show more content†¦Discussion 2, Barriers to Entry. Analyze the major barriers for entry and exit into the airline industry. Explain how each barrier can foster either monopoly or oligopoly. What barriers, if any, do you feel give rise to monopoly that will allow the government to become involved to protect consumers? Week 5 Discussion 1. Transfers. Why would cash transfers typically be preferred by recipients over in-kind transfers? What are the pros and cons of each from a government perspective? Respond to at least two of your classmates. Discussion 2, Tariffs and Quotas. Who gains and who loses from a tariff? How do the effects of tariffs differ from the effects of quotas? If you were a small country, what would you rather utilize? Focus of the Final Paper: Market Structures You have been hired as a consultant by your local mayor to look at the various market structures. Your role is to provide analysis and answers to these important questions that will help the mayor understand the structures of many of the businesses in his city: 1. Describe each market structure discussed in the course (perfect competition, monopolistic competition, oligopoly, and monopoly) and discuss two of the market characteristics of each market structure. 2. Identify one real-life example of a market structure in your local city and relate your example to each of the characteristics of the